Friday, October 24, 2014 2pm to 3pm
About this Event
Greenville, NC 27858-4353
The Department of Chemistry will host speaker, Jamie DeWitt, Ph.D. from the Department of Pharmacology and Toxicology, East Carolina University Brody School of Medicine. She will be sharing her exciting research with you.
ABSTRACT - Perfluorooctanoic acid (PFOA) is a perfluoroalkyl substance (PFAS) that has been used as a surfactant in the production of myriad industrial and consumer products. However, its presence in the environment and demonstrated multisystem toxicity in several animal models led to a voluntary phase-out by several US manufacturers. A potential replacement for PFOA is undecafluoro-2-methyl-3-oxahexanoic acid (U2M3). Exposure to PFOA and related PFAS suppress T cell-dependent antibody responses (TDAR) in mice without observable impacts on T or B cell numbers. This study evaluated TDAR in mice exposed to U2M3. Doses were based on publicly available information indicating a NOAEL of 10 mg/kg/d for evidence of regenerative anemia. Adult male and female C57BL/6 mice were gavaged with 0, 1, 10, or 100 mg/kg/d of U2M3 per BW for 28d. After 24d, mice were immunized with a T cell-dependent antigen. On day 29, animals were euthanized and sera were evaluated for IgM TDAR. U2M3 exposure did not alter BW or lymphoid organ weights; liver weights were increased in males and females at 10 (~40%) and 100 (~140%) mg/kg U2M3 relative to controls. Mean IgM serum titers of males dosed with 100 mg/kg were statistically suppressed by 10% relative to controls; female responses did not vary statistically from controls. Splenic CD45R B cell numbers were suppressed in males exposed to 100 (10%) mg/kg, but no other changes in numbers of splenic lymphocytes were observed for males or females at any dose. Serum concentrations for 100 mg/kg after 28d of exposure were 21595 ng/mL for males and 7132 ng/mL for females, which may partially explain the lack of TDAR suppression in females. However the relevance of the TDAR response and serum concentrations to organisms environmentally-exposed is unknown as environmental concentrations of this potentially emerging compound also are unknown.
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